Edward L. D'Antonio, Ph. D.
Assistant Professor of Biochemistry and Structural Biology
University of South Carolina Beaufort
Science and Technology Building, Room 108
One University Boulevard, Bluffton, SC 29909
Office: 843-208-8101 Lab: 843-208-8167 Fax: 843-208-8294
Structural Biology, Biochemistry, Chagas' Disease, and Medicinal Chemistry.
My team consists of undergraduate researchers that utilize a variety of techniques, including: analytical chemistry, biotechnology, enzymology, structural biology, and synthetic chemistry. I use the structure-based drug design methodology to develop new classes of competitive inhibitors for enzyme drug-targets found in parasites of trypanosomatid diseases. These diseases include Chagas' disease (Trypanosoma cruzi), human African trypanosomiasis (Trypanosoma brucei), and leishmaniasis (Leishmania spp.). I focus on pentose phosphate pathway enzymes, which are known to be critically important for the survival of these parasites. Since human cells usually have similar enzymes of these drug-targets, our drug designs are created that give rise to parasite selectivity. Another goal of my research is to optimize existing inhibitor designs by increasing molecular interactions within the inhibitor's binding site.
- B.S. in Chemistry, Millersville University (2003)
Advisor: Dr. Lyman H. Rickard
- Ph.D. in Chemistry, North Carolina State University (2010)
Advisor: Dr. Edmond F. Bowden
- Postdoctoral Fellowship in Structural Biology, University of Pennsylvania (2010-2013)
Advisor: Dr. David W. Christianson
- Principles of Biochemistry I
- Quantitative Analysis
- Forensic Techniques and Data Analysis
- General Chemistry I & II
- Seminar in Biochemistry Research (Survey of Drug Discovery Methodologies)
About the Group
Publications and Patents
 D'Antonio, E. L. and Cordeiro, A. T. (2016) Inhibitors of glucose kinases, along
with methods of their formation and use. U.S. Provisional Patent Application (submitted).
 D'Antonio, E. L. (2016) Cysteine-modifying substrate analogue inhibitors of ribose
5-phosphate isomerase for parasitic diseases, along with methods of their formation
and use. U.S. Provisional Patent Application No. 15/360,139.
More about this technology: http://techfinder.sc.edu/technology/22324
 D'Antonio, E. L., Deinema, M. S., Kearns, S. P., Frey, T. A., Tanghe, S., Perry, K.,
Roy, T. A., Gracz, H. S., Rodriguez, A., and D’Antonio, J. (2015) Structure-based
approach to the identification of a novel group of selective glucosamine analogue
inhibitors of Trypanosoma cruzi glucokinase. Mol. Biochem. Parasitol. 204, 64-76.
 D'Antonio, E. L. and D'Antonio, J. (2014) Therapeutic monosaccharide-based
inhibitors of hexokinase and glucokinase for parasitic diseases, along with methods
of their formation and use. U.S. Provisional Patent Application No. 62/082,861.
More about this technology: http://techfinder.sc.edu/technology/17947
 Taratula, O., Bai, Y., D'Antonio, E. L., and Dmochowski, I. J. (2014) Enantiopure
cryptophane-129Xe nuclear magnetic resonance biosensors targeting
carbonic anhydrase. Supramol. Chem. 1-8, DOI: 10.1080/10610278.2014.906601.
 Chen, M., Al-lami, N., Janvier, M., D'Antonio, E. L., Faraldos, J. A., Cane,
D. E., Allemann, R. K., and Christianson, D. W. (2013) Mechanistic insights
from the binding of substrate and carbocation intermediate analogues to aristolochene
synthase. Biochemistry, 52, 5441-5453.
 D'Antonio, E. L., Ullman, B., Roberts, S. C., Gaur Dixit, U., Wilson, M. E.,
Hai, Y., and Christianson, D. W. (2013) Crystal structure of arginase from
Leishmania mexicana and implications for the inhibition of polyamine biosynthesis
in parasitic infections. Arch. Biochem. Biophys., 535, 163-176.
 Genshaft, A., Moser, J-A., D'Antonio, E. L., Bowman, C., and Christianson, D. W.
(2013) Energetically unfavorable amide conformations for N6-acetyllysine side chains
in refined protein structures. Proteins: Struct., Funct., Bioinf., 81, 1051-1057.
 D'Antonio, E. L., Chen, T. K., Turner, A. H., Santiago-Capeles, L., and
Bowden, E. F. (2013) Voltammetry of dehaloperoxidase on self-assembled monolayers:
reversible adsorptive immobilization of a globin. Electrochem. Commun., 26, 67-70.
 D'Antonio, E. L., Hai, Y., and Christianson, D. W. (2012) Structure and function of
non-native metal clusters in human arginase I. Biochemistry, 51, 8399-8409.
 D'Antonio, E. L. and Christianson, D. W. (2012) Binding of the unreactive substrate
analog L-2-amino-3-guanidinopropionic acid (dinor-L-arginine) to human arginase I.
Acta Crystallogr., F68, 889-893.
 D'Antonio, E. L., Bowden, E. F., and Franzen, S. (2012) Thin-layer
spectroelectrochemistry of the Fe(III)/Fe(II) redox reaction of dehaloperoxidase-hemoglobin.
J. Electroanal. Chem., 668, 37-43.
 D'Antonio, E. L., D'Antonio, J., de Serrano, V., Gracz, H., Thompson, M. K.,
Ghiladi, R. A., Bowden, E. F., and Franzen, S. (2011) Functional consequences
of the creation of an Asp-His-Fe triad in a 3/3 globin. Biochemistry, 50, 9664-9680.
 D'Antonio, E. L. and Christianson, D. W. (2011) Crystal structures of complexes
with cobalt-reconstituted human arginase I. Biochemistry, 50, 8018-8027.
 D'Antonio, J., D'Antonio, E. L., Thompson, M. K., Bowden, E. F., Franzen, S.,
Smirnova, T., Ghiladi, R. A. (2010) Spectroscopic and mechanistic investigations
of dehaloperoxidase B from Amphitrite ornata. Biochemistry, 49, 6600-6616.
 de Serrano, V., Davis, M. F., Gaff, J. F., Zhang, Q., D'Antonio, E. L., Bowden, E. F.,
Rose, R., and Franzen, S. (2010) X-ray structure of the metcyano form of
dehaloperoxidase from Amphitrite ornata. Acta Crystallogr., D66, 770-782.